1.Add reagent to 100ml water sample,after dissolving, incubate at 36°C for 24h 2. Interpretation of results: colorless = negative Yellow = positive for total coliforms Yellow + fluorescence = Escherichia coli positive. QUANTlTATlVE DETECTlON3. Use the program-controlled quantitative sealing machine to seal the quantitative detection disc (quantitative well plate) for sealing and incubate at 36°C for 24h Heat-resistant coliform/fecal coliform culture at 44.5°C for 24h is yellow and positive 4. Interpretation of results: colorless = negative Yellow checkered = positive total coliforms Yellow + fluorescent grid = Escherichia coli positive reference MPN table count1. Add the reagents to the water sample and mix well 2. Pour into 51-well quantitative detection plate (quantitative well plate) or 97-well quantitative detection plate (quantitative well plate)