| Summary | Used to detect specific antibodies against avian influenza virus (AIV) in serum |
| Principle | Avian influenza antibody Elisa kit is used to detect specific antibody against Avian influenza Virus (AIV) in serum, for monitoring antibody after AIV immune and serological diagnosis of infection in Avian. |
| Detection Targets | Avian Influenza Antibody |
| Sample | Serum |
| Quantity | 1 kit = 192 Test |
| Stability and Storage | 1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit. |
| Reagent | Volume 96 Tests/192Tests | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Instruction | 1 pcs |
This kit use block ELISA method, AIV antigen is pre-coated on microplate. When testing, add diluted serum sample, after incubation, if there is AIV specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-AIV monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.