| Summary | used to detect Avian leukosis P27 antigen in avian blood, feces, cloaca, and egg white. |
| Principle | Avian Leukosis (AL) P27 antigen Elisa kit is used to detect Avian leukosis P27 antigen in avian blood, feces, cloaca, and egg white.
|
| Detection Targets | Avian Leukosis (AL) P27 antigen |
| Sample | Serum |
| Quantity | 1 kit = 192 Test |
| Stability and Storage | 1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit. |
| Reagent | Volume 96 Tests/192Tests | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Instruction | 1 pcs |
The kit uses a sandwich ELISA method,pured anti-avian leukocyte P27 monoclonal antibody is pre-coated on enzyme micro-well strips.In the test, the antigen in the sample is bound to the antibody on the coated plate, after washing to remove the unbound antigen and other components, the enzyme monoclonal antibody is added to specifically bind to the antigen-antibody complex on the test plate. then washing, the unbound enzyme conjugate is removed, TMB substrate solution is added to the microplate, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample. Add stop solution, After reaction, the absorbance A value in the reaction well is measured by a wavelength of 450 nm.