Bovine Tuberculosis Antibody ELISA Kit

Summary	Detection of specific Bovine Tuberculosis(BTB) antibody
Principle	Bovine Tuberculosis(BTB) antibody Elisa test kit can be used to detect Bovine Tuberculosis antibody in serum or plasma of Bovine.
Detection Targets	Bovine Tuberculosis(BTB) antibody
Sample	Serum
Quantity	1 kit = 192 Test
Stability and Storage	1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

Mycobacterium bovis is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to Mycobacterium tuberculosis, the bacterium which causes tuberculosis in humans. M. bovis can jump the species barrier and cause tuberculosis-like infection in humans and other mammals.

Reagent

Volume

96 Tests/192Tests

Antigen coated microplate

1ea/2ea

Negative Control

2.0ml

Positive Control

1.6ml

Sample diluents

100ml

Washing solution (10X concentrated)

100ml

Enzyme conjugate

11/22ml

Substrate

11/22ml

Stopping solution

15ml

Adhesive plate sealer

2ea/4ea

serum dilution microplate

1ea/2ea

Instruction

1 pcs

This kit use indirect ELISA method, pured BTB antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is BTB specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme conjugate, discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample.

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