Brucellosis Antibody Competitive Elisa Kit

Products Details

Summary  Detection of specific Antibody of Foot and MouthDisease Type  The BRU
Principle

The BRU antibody ELISA test kit use to detection of Brucellosis antibodies in the serum of pigs, cattle, sheep and goat .

Detection Targets The BRU antibody
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Brucellosisis a highly contagious zoonosis caused by ingestion of unpasteurized milk or undercooked meat from infected animals, or close contact with their secretions.  It is also known as undulant fever, Malta fever, and Mediterranean fever.

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate
 

1ea/2ea

2
 Negative Control
 

2ml

3
 Positive Control
 

1.6ml

4
 Sample diluents
 

100ml

5
Washing solution (10X concentrated)
 

100ml

6
 Enzyme conjugate
 

11/22ml

7
 Substrate
 

11/22ml

8
 Stopping solution
 

15ml

9
Adhesive plate sealer
 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 

The BRU antibody ELISA test kit use to detection of Brucellosis antibodies in the serum of pigs, cattle, sheep and goat .

This kit use competitive ELISA method to pre-coated BRU antigens on microplate wells. When testing, add diluted serum sample and enzyme labled anti-BRU monoclonal antibody, after incubation, if there have BRU antibody, it will combine with the pre-coated antigen,  antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample

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