Brucellosis antibody indirect Elisa kit

Products Details

Summary  Detection of specific Antibody of Foot and MouthDisease Type  The BRU
Principle

Principle of the Test  Brucellosis(Bru) antibody Elisa test kit can be used to detect Brucellosis antibody in serum or plasma of Bovine and Pig.

Detection Targets The BRU antibody
Sample  Serum 
Quantity 1 kit = 192 Test
   Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Brucellosis is a highly contagious zoonosis caused by ingestion of unpasteurized milk or undercooked meat from infected animals, or close contact with their secretions. It is also known as undulant fever, Malta fever, and Mediterranean fever.

 

 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate
 

1ea/2ea

2
 Negative Control
 

2ml

3
 Positive Control
 

1.6ml

4
 Sample diluents
 

100ml

5
Washing solution (10X concentrated) 
 

100ml

6
 Enzyme conjugate
 

11/22ml

7
 Substrate
 

11/22ml

8
 Stopping solution
 

15ml

9
Adhesive plate sealer
 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 This kit use indirect ELISA method, pured Bru antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Bru specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme conjugate, discard the uncombined enzyme conjugate with  washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample.

Contact us

Please feel free to give your inquiry in the form below We will reply you in 24 hours