Eggs Drop Syndrome1976 virus Antibody ELISA Ki

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Products Details

Summary   Use to detect specific antibody against EDS76 in serum qualitatively.
Principle

The Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit is use to detect specific antibody against EDS76 in serum qualitatively. For monitoring antibody after EDS76 immune and serological diagnostic of infection in Avian.
Detection Targets  Antibody against EDS76 in serum
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Egg Drop Syndrome (EDS-76) is an acute infectious disease caused by adenovirus group III of the Adenoviridae Avian Virus genus with hemagglutination. In some chicken farms, the mass egg production of chickens suddenly decreased, and deformed eggs such as soft-shell eggs, shell-less eggs, and thin-shell eggs were produced at the same time. The whole course of the disease lasts for 5-6 weeks, after which the egg production rate gradually rises, but it is difficult to reach the level before the decline
 

Reagent

Volume

96 Tests/192Tests
1
Antigen coated microplate
 

1ea/2ea
2
 Negative Control
 

2.0ml
3
 Positive Control
 

1.6ml
4
 Sample diluents
 

100ml
5
Washing solution (10X concentrated)
 

100ml
6
 Enzyme conjugate
 

11/22ml
7
 Substrate
 

11/22ml
8
 Stopping solution
 

15ml
9
Adhesive plate sealer
 

2ea/4ea
10 serum dilution microplate

1ea/2ea
11  Instruction

1 pcs
 This kit use indirect ELISA method, pured EDS76 antigen is pre-coated on enzyme micro-well strips.Whentesting,adddilutedserumsample,afterincubation,ifthereisEDS76virusspecificantibody,it will combine with the pre-coatedantigen,discardtheuncombined antibody and other components with washing; then add enzyme labled anti-EDS76 virus monoclonal antibody, then combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution  to stop the reaction.

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