Foot and Mouth Disease Type A Antibody ELISA Test Kit

Products Details

Summary Detection of specific Foot and Mouth Disease Type A antibody
Principle

The FMD Type A antibody ELISA test kit use to detection of foot-and-mouth disease virus antibodies in the serum of pigs, cattle, sheep and goat for the evaluate of FMD vaccine immunity.

Detection Targets Foot and Mouth Disease Type A  Antibody
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Foot-and-mouth disease virus (FMDV) is the pathogen that causes foot-and-mouth disease. It is a picornavirus, the prototypical member of the genus Aphthovirus. The disease, which causes vesicles (blisters) in the mouth and feet of cattle, pigs, sheep, goats, and other cloven-hoofed animals is highly infectious and a major plague of animal farming.
 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate
 

1ea/2ea

2
 Negative Control
 

2.0ml

3
 Positive Control
 

1.6ml

4
 Sample diluents
 

100ml

5
Washing solution (10X concentrated)
 

100ml

6
 Enzyme conjugate
 

11/22ml

7
 Substrate
 

11/22ml

8
 Stopping solution
 

15ml

9
Adhesive plate sealer
 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 This kit use competitive ELISA method to pre-coated foot-and-mouth disease virus antigens on microplate wells. When testing, add diluted serum sample and anti-FMD Ab, after incubation, if there have FMD antibody, it will combine with the pre-coated antigen, antibody in sample block the combination of anti-FMD antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.Foot-and-mouth disease virus occurs in seven major serotypes: O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1. These serotypes show some regionality, and the O serotype is most common.

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