Foot and Mouth Disease Type O Ab ELISA Test Kit

Products Details

Summary Detection of specific  Foot and Mouth antibody Type O
Principle

The FMD Type Asia I antibody ELISA test kit use to detection of foot-and-mouth disease virus antibodies in the serum of pigs, cattle, sheep and goat for the evaluate of FMD vaccine immunity.

Detection Targets Foot and Mouth antibody Type O
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Foot-and-mouth disease virus (FMDV) is the pathogen that causes foot-and-mouth disease.[1] It is a picornavirus, the prototypical member of the genus Aphthovirus. The disease, which causes vesicles (blisters) in the mouth and feet of cattle, pigs, sheep, goats, and other cloven-hoofed animals is highly infectious and a major plague of animal farming
 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate
 

1ea/2ea

2
 Negative Control
 

2ml

3
 Positive Control
 

1.6ml

4
 Sample diluents
 

100ml

5
Washing solution (10X concentrated) 
 

100ml

6
 Enzyme conjugate
 

11/22ml

7
 Substrate
 

11/22ml

8
 Stopping solution
 

15ml

9
Adhesive plate sealer
 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 ThiskituseindirectELISAmethod,puredFMDVantigenispre-coated on enzyme micro-well strips. When testing,adddiluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme conjugate, discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample.

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