H5 Subtype Avian Influenza Antibody ELISA Kit

Products Details

Summary Used to detect specific antibody against Avian influenza Virus (AIV-H5) in serum
Principle

H5 subtype avian influenza antibody Elisa kit is used to detect specific antibody against Avian influenza Virus (AIV-H5) in serum, for monitoring antibody after AIV-H5 immune and serological diagnosis of infection in Avian.

 

Detection Targets H5 subtype avian influenza antibody
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Avian influenza, known informally as avian flu or bird flu, is a variety of influenza caused by viruses adapted to birds. The type with the greatest risk is highly pathogenic avian influenza (HPAI). Bird flu is similar to swine flu, dog flu, horse flu and  human flu as an illness caused by strains of influenza viruses that have adapted to a specific host.  Out of the three types of influenza viruses (A, B, and C), influenza A virus is a zoonotic infection with a natural reservoir almost  entirely in birds.Avian influenza, for most purposes, refers to the influenza A virus.
 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate
 

1ea/2ea

2
 Negative Control
 

2.0ml

3
 Positive Control
 

1.6ml

4
 Sample diluents
 

100ml

5
Washing solution (10X concentrated)
 

100ml

6
 Enzyme conjugate
 

11/22ml

7
 Substrate
 

11/22ml

8
 Stopping solution
 

15ml

9
Adhesive plate sealer
 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 This kit use block ELISA method, AIV-H5 antigen is pre-coated on microplate. When testing, add diluted serum sample, after incubation, if there is AIV-H5 specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-AIV-H5 monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample

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