| Summary | Detection of specific Antibody of Flu A immune and serological diagnosis of infection in Avian, swine and Equus. |
| Principle | Influenza A antibody Elisa kit is used to detect specific antibody against Influenza A Virus (Flu A) in serum, for monitoring antibody after Flu A immune and serological diagnosis of infection in Avian, swine and Equus.
|
| Detection Targets | Influenza A antibody |
| Sample | Serum |
| Quantity | 1 kit = 192 Test |
| Stability and Storage | 1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit. |
| Reagent | Volume 96 Tests/192Tests | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Instruction | 1 pcs |
This kit use block ELISA method, FluA antigen is pre-coated on microplate. When testing, add diluted serum sample, after incubation, if there is Flu A specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Flu A monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.