Newcastle Disease Antibody ELISA Kit

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Products Details

Summary  Detection of specific Antibody of Newcastle diseasespecific antibodies
Principle

Newcastle disease antibody Elisa kit is used to detect specific antibody against Newcastle disease Virus (NDV)  in serum, for monitoring antibody after NDV immune and serological diagnosis of infection in Avian.

 
Detection Targets Newcastle disease antibody
Sample  Serum  
Quantity 1 kit = 192 Test
    Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

   
 Newcastle disease is a contagious viral avian disease affecting many domestic and wild bird species; it is transmissible to humans.Though it can infect humans, most cases are non-symptomatic; rarely it can cause a mild fever and influenza-like symptoms and/or conjunctivitis in humans. Its effects are most notable in domestic poultry due to their high susceptibility and the potential for severe impacts of an epizootic on the poultry industries. It is endemic to many countries. No treatment for it s known, but the use of prophylactic vaccines and sanitary measures reduces the likelihood of outbreaks.
 

Reagent

Volume

96 Tests/192Tests
1
Antigen coated microplate
 

1ea/2ea
2
 Negative Control
 

2.0ml
3
 Positive Control
 

1.6ml
4
 Sample diluents
 

100ml
5
Washing solution (10X concentrated)
 

100ml
6
 Enzyme conjugate
 

11/22ml
7
 Substrate
 

11/22ml
8
 Stopping solution
 

15ml
9
Adhesive plate sealer
 

2ea/4ea
10 serum dilution microplate

1ea/2ea
11  Instruction

1 pcs
 This kit use block ELISA method, NDV antigen is pre-coated on microplate. When testing, add diluted serum sample, after incubation, if there is NDV specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-NDV monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample. 

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